Photomedicine and Laser Surgery
Green Light Emitting Diode Irradiation Enhances Fibroblast Growth Impaired by High Glucose Level
Apr 2005, Vol. 23, No. 2 : 167 -171

Elke M. Vinck, P.T.
Department of Rehabilitation Sciences and Physiotherapy, Ghent University, 9000 Ghent, Belgium.
Barbara J. Cagnie, P.T.
Department of Human Anatomy, Embryology, Histology and Medical Physics, Ghent University, 9000 Ghent, Belgium.
Maria J. Cornelissen, Ph.D.
Department of Rehabilitation Sciences and Physiotherapy, Ghent University, 9000 Ghent, Belgium.
Heidi A. Declercq
Department of Human Anatomy, Embryology, Histology and Medical Physics, Ghent University, 9000 Ghent, Belgium.
Dirk C. Cambier, Ph.D.
Department of Rehabilitation Sciences and Physiotherapy, Ghent University, 9000 Ghent, Belgium.

Background and Objective: The chronic metabolic disorder diabetes mellitus is an important cause of morbidity and mortality due to a series of common secondary metabolic complications, such as the development of severe, often slow healing skin lesions. In view of promoting the wound-healing process in diabetic patients, this preliminary in vitro study investigated the efficacy of green light emitting diode (LED) irradiation on fibroblast proliferation and viability under hyperglycemic circumstances. Materials and Methods: To achieve hyperglycemic circumstances, embryonic chicken fibroblasts were cultured in Hanks' culture medium supplemented with 30 g/L glucose. LED irradiation was performed on 3 consecutive days with a probe emitting green light (570 nm) and a power output of 10 mW. Each treatment lasted 3 min, resulting in a radiation exposure of 0.1 J/cm2. Results: A Mann–Whitney U test revealed a higher proliferation rate (p = 0.001) in all irradiated cultures in comparison with the controls. Conclusion: According to these results, the effectiveness of green LED irradiation on fibroblasts in hyperglycemic circumstances is established. Future in vivo investigation would be worthwhile to investigate whether there are equivalent positive results in diabetic patients.

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